2-1201-002

Strep-Tactin® Sepharose® resin

Name: Strep-Tactin® Sepharose® resin
Brand: IBA Lifesciences
SKU: 2-1201-002
Price: 125.0 EUR
Availability: InStock

50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins

Please note that this product will be discontinued and replaced by Strep-Tactin® 4Flow® high capacity resin. Strep-Tactin® Sepharose® resin will still be available until 31^st October 2024 or as long as stock lasts. Check out the features of the new resin here.

Strep-Tactin® Sepharose® 50 % suspension can be used for the purification of Strep-tag®II or Twin-Strep-tag® fusion proteins. The resin is particularly suited for the purification of proteins with multiple tags, such as multimers, that cannot be eluted from Strep-Tactin®XT resins because of the increased affinity. It can be used for the purification of large proteins, due to the low percentage of agarose. Strep-Tactin® irreversibly binds biotin, so if free biotin is expected use BioLock. The suspension format allows the packing of gravity flow columns with custom resin volumes. For a 1 ml resin bed volume, use 2 ml 50 % suspension.

Strep-Tactin® Sepharose® is not compatible with HPLC/FPLC due to a low pressure stability.

Specifications

Bead Size:	45 - 165 µm
Biotin Binding Capacity:	> 300 nmol/ml resin
Exclusion Limit:	3 x 10^7
Form:	50% suspension
Possible Application:	Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Specificity:	Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin
Strep-tag®II Binding Capacity:	3 mg/ml
Support:	4% agarose