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10x Buffer E contains desthiobiotin and is a 10x concentrated elution buffer for Strep-Tactin® resins. Desthiobiotin is a modified form of biotin that displaces Strep-tag®II and Twin-Strep-tag® fusion proteins from the biotin binding pocket of Strep-Tactin®.
Please note that 10x Buffer E is not applicable for elution from Strep-Tactin®XT resins.

Specifications

Concentration: 10x concentrated
Form: Solution
Possible Application: Competitive elution of Strep-tag®II and Twin-Strep-tag® fusion proteins from Strep-Tactin® resins

Shipping information

Storage: 2-8 °C
Stability: 12 months after shipping
Shipping: Room temperature

Accessories

Strep-Tactin® Sepharose® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity FPLC column
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® MacroPrep® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® 4Flow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins - New product! Receive 10% introductory discount* until 31.12.24.
Strep-Tactin® Superflow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® 4Flow® high capacity FPLC column
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins - New product! Receive 10% introductory discount* until 31.12.24.
Strep-Tactin® 4Flow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins - New product! Receive 10% introductory discount* until 31.12.24.

Related Products

Strep-Tactin® Buffer Set
Strep-Tactin® purification buffer set
Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer
Buffer R (10x)
Strep-Tactin® regeneration buffer with HABA