2-1206-025

Strep-Tactin® Superflow® resin

Name: Strep-Tactin® Superflow® resin
Brand: IBA Lifesciences
SKU: 2-1206-025
Availability: InStock

50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins

Please note that this product will be discontinued and replaced by Strep-Tactin® 4Flow® high capacity resin. Strep-Tactin® Superflow® resin will still be available until 31^st October 2024 or as long as stock lasts.
Check out the features of the new resin here.

Strep-Tactin® Superflow® is suitable for affinity chromatography-based purification of strep-tagged fusion proteins from cell culture media, cell lysates or other mixed protein preparations. It is optimized for column affinity chromatography and recommended for proteins <90 kDa.

The resin consists out of highly cross-linked agarose beads coupled to Strep-Tactin®, a streptavidin variant that binds to proteins fused to a Strep-tag®II or a Twin-Strep-tag®. This purification system delivers an outstanding target purity (>95%) without tedious optimization steps due to the specific binding of tagged proteins and a subsequent targeted elution. Resin regeneration is possible, enabling multiple purification cycles.

Please note that biotinylated proteins also bind to Strep-Tactin® Superflow®. If biotinylated proteins are expected, use BioLock. Furthermore, this resin is a 50% suspension, so for packing e.g. a 1 ml gravity column, 2 ml of resin is required.

Specifications

Bead Size:	60 - 160 µm spherical
Biotin Binding Capacity:	> 300 nmol/ml resin
Exclusion Limit:	6 x 10^6
Form:	50% suspension
Possible Application:	Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Specificity:	Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin
Strep-tag®II Binding Capacity:	3 mg/ml
Support:	6% agarose, crosslinked