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Strep-Tactin® Buffer Set contains all buffers needed for the purification of Strep-tag®II or Twin-Strep-tag® fusion proteins and subsequent resin regeneration.
10x Buffer W is a 10x concentrated wash buffer for Strep-Tactin® and Strep-Tactin®XT resins. During protein purification, it removes unbound molecules from the affinity chromatography matrix. 10x Buffer E is a 10x concentrated Strep-Tactin® elution buffer containing desthiobiotin. Desthiobiotin is a modified form of biotin that displaces Strep-tag®II and Twin-Strep-tag® proteins from the modified biotin-binding pocket of Strep-Tactin®. As a result, a mild and highly specific competitive elution occurs. Please note that 10x Buffer E is not applicable for elution from Strep-Tactin®XT resins. 10x Buffer R is a 10x concentrated Strep-Tactin® regeneration buffer containing HABA (2-[4'-hydroxy-benzeneazo]benzoic acid). HABA displaces desthiobiotin from the modified biotin-binding pocket of Strep-Tactin® Sepharose®, Strep-Tactin® Superflow® and Strep-Tactin® Superflow® high capacity after elution (not suitable for regeneration of Strep-Tactin® 4Flow® high capacity). Upon binding of HABA to Strep-Tactin®, the yellow buffer turns red, indicating regeneration and column activity.
10x Buffer W is a 10x concentrated wash buffer for Strep-Tactin® and Strep-Tactin®XT resins. During protein purification, it removes unbound molecules from the affinity chromatography matrix. 10x Buffer E is a 10x concentrated Strep-Tactin® elution buffer containing desthiobiotin. Desthiobiotin is a modified form of biotin that displaces Strep-tag®II and Twin-Strep-tag® proteins from the modified biotin-binding pocket of Strep-Tactin®. As a result, a mild and highly specific competitive elution occurs. Please note that 10x Buffer E is not applicable for elution from Strep-Tactin®XT resins. 10x Buffer R is a 10x concentrated Strep-Tactin® regeneration buffer containing HABA (2-[4'-hydroxy-benzeneazo]benzoic acid). HABA displaces desthiobiotin from the modified biotin-binding pocket of Strep-Tactin® Sepharose®, Strep-Tactin® Superflow® and Strep-Tactin® Superflow® high capacity after elution (not suitable for regeneration of Strep-Tactin® 4Flow® high capacity). Upon binding of HABA to Strep-Tactin®, the yellow buffer turns red, indicating regeneration and column activity.
| Concentration: | 10x concentrated |
|---|---|
| Form: | Solution |
| Kit Content: | 100 ml 10x Buffer W, 25 ml 10x Buffer E, 100 ml 10x Buffer R |
| Possible Application: | Protein purification with Strep-Tactin® resins |
| Storage: | 2-8 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
Accessories
Strep-Tactin® Sepharose® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Sepharose® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity FPLC column
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® MacroPrep® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
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