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Strep-Tactin® MacroPrep® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® MacroPrep® consists of polymethacrylate, a synthetic material coupled with the streptavidin variant Strep-Tactin® and is applicable for purification of Strep-tag®II or Twin-Strep-tag® fusion proteins. In contrast to agarose resins, plant cell components such as chlorophyll are not unspecifically bound by this resin, which makes Strep-Tactin® MacroPrep® the favorable alternative.
The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography, rather than for batch purification. To pack a column with 1 ml resin bed volume, 2 ml 50% suspension are necessary.
The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography, rather than for batch purification. To pack a column with 1 ml resin bed volume, 2 ml 50% suspension are necessary.
| Bead Size: | 50 µm |
|---|---|
| Biotin Binding Capacity: | > 300 nmol/ml resin |
| Exclusion Limit: | 1 x 10^6 |
| Form: | 50% suspension |
| Possible Application: | Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins |
| Specificity: | Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin |
| Strep-tag®II Binding Capacity: | 3 mg/ml |
| Support: | Polymethacrylate |
| Storage: | 2-8 °C |
|---|---|
| Stability: | 6 months after shipping |
| Shipping: | Room temperature |
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