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Strep-Tactin®XT 4Flow® 50% suspension is coupled to the high affinity ligand Strep-Tactin®XT and can be used for the purification of Strep-tag®II and Twin-Strep-tag® proteins. The resin offers outstanding target purity (>95%), a high binding affinity and a low agarose concentration. It is recommended for use with all protein classes, especially low abundant or challenging proteins. For proteins with an increased affinity, like those with multiple tags or multimers, we recommend using a Strep-Tactin® resin. The suspension format allows the packing of custom gravity flow columns. For a 1 ml resin bed volume, use 2 ml 50 % suspension.
A great pressure stability enables the use of Strep-Tactin®XT 4Flow® in FPLC setups.

Please review the details of our other resins if you have other needs for your purification.

Specifications

Bead Size: 50 - 150 µm spherical
Form: 50% suspension
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Protein Binding Capacity: 11 mg/ml resin
Specificity: Twin-Strep-tag® and Strep-tag®II
Support: 4% agarose, crosslinked

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer
Buffer BXT (10x)
Strep-Tactin®XT elution buffer
Biotin
Biotin powder for preparation of Strep-Tactin®XT elution buffer
Buffer XT-R
Strep-Tactin®XT regeneration buffer
Strep-Tactin®XT Buffer Set
Strep-Tactin®XT purification buffer set

Related Products

MagStrep® Strep-Tactin®XT beads
Magnetic beads (5% suspension) to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity Spin Column Kit
For rapid purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Empty Spin Columns
For rapid protein purification with Strep-Tactin®XT 4Flow® high capacity
Strep-Tactin®XT 4Flow® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® FPLC column
FPLC column to purify Strep-Tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity FPLC column
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins