2-5011-005

Strep-Tactin®XT 4Flow® column

Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins

€164.00*

Content

Strep-Tactin®XT 4Flow® columns contain a 4% agarose coupled with the streptavidin variant Strep-Tactin®XT and are applicable for purification of Strep-tag®II or Twin-Strep-tag® fusion proteins via gravity flow. Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (> 95%), and highest binding affinity, the resin is recommended for all protein classes, especially for low abundant or challenging proteins. The low concentrated agarose allows for the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets. In addition, Strep-Tactin®XT 4Flow® enables the application of denaturing agents such as urea.
To reduce the waiting period during the sample and buffer application, the use of the application aid WET FRED is recommended.

Specifications

Bead Size: 50 - 150 µm spherical
Dynamic Protein Binding Capacity: 5 mg/ml resin
Form: Pre-packed gravity flow column
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Specificity: Twin-Strep-Tag® and Strep-tag®II
Support: 4% agarose, crosslinked

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

WET FRED Set
Application aid for large volumes of lysates or culture supernatants
10x Buffer W
Strep-Tactin® and Strep-Tactin®XT wash buffer
10x Buffer BXT
Strep-Tactin®XT elution buffer
Biotin
Biotin powder for preparation of Strep-Tactin®XT elution buffer
Buffer XT-R
Strep-Tactin®XT regeneration buffer
Strep-Tactin®XT Buffer Set
Strep-Tactin®XT purification buffer set

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