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10x Buffer BXT is a 10x concentrated Strep-Tactin®XT elution buffer. It has to be diluted before use. Biotin reversibly binds to Strep-Tactin®XT, which allows the elution of Strep-tag®II or Twin-Strep-tag® fusion proteins.
Please note that biotin binds irreversibly to Strep-Tactin®. Therefore, 10x Buffer BXT is not applicable for elution of Strep-tag®II or Twin-Strep-tag® proteins from Strep-Tactin® resins, since they cannot be regenerated after use.

Specifications

Concentration: 10x concentrated
Form: Solution
Possible Application: Competitive elution of Strep-tag®II and Twin-Strep-tag® fusion proteins from Strep-Tactin®XT resins

Shipping information

Storage: 2-8 °C
Stability: 12 months after shipping
Shipping: Room temperature

Accessories

Strep-Tactin®XT 4Flow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® FPLC column
FPLC column to purify Strep-Tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity FPLC column
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins

Related Products

Biotin
Biotin powder for preparation of Strep-Tactin®XT elution buffer
Strep-Tactin®XT Buffer Set
Strep-Tactin®XT purification buffer set
Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer
Buffer XT-R
Strep-Tactin®XT regeneration buffer