10x Buffer BXT is a 10x concentrated Strep-Tactin®XT elution buffer. It has to be diluted before use. Biotin reversibly binds to Strep-Tactin®XT, which allows the elution of Strep-tag®II or Twin-Strep-tag® fusion proteins.
Please note that biotin binds irreversibly to Strep-Tactin®. Therefore, 10x Buffer BXT is not applicable for elution of Strep-tag®II or Twin-Strep-tag® proteins from Strep-Tactin® resins, since they cannot be regenerated after use.
Concentration: | 10x concentrated |
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Form: | Solution |
Possible Application: | Competitive elution of Strep-tag®II and Twin-Strep-tag® fusion proteins from Strep-Tactin®XT resins |
Storage: | 2-8 °C |
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Stability: | 12 months after shipping |
Shipping: | Room temperature |