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MagStrep® Strep-Tactin®XT beads can be used for the purification of strep-tagged fusion proteins in batch or high-throughput format.
For cell purification we offer specific magnetic beads with a smaller diameter (1µm) and for BioID targets we recommend our MagStrep® Strep-Tactin® beads.
MagStrep® Strep-Tactin®XT beads show low non-specific protein binding due to their agarose surface and high specificity of Strep-Tactin®XT towards Strep-tag. This results in outstanding target protein purity and makes the MagStrep® Strep-Tactin®XT beads the ideal tool for protein isolation and complex identification via pull-downs. MagStrep® Strep-Tactin®XT beads are suitable for fast small-scale purifications in reaction tubes as well as 96-well plates.
The beads can be easily and swiftly separated with a magnetic separator. This eliminates the need for centrifugation and reduces the risk of disturbing the pellet or loss of beads within the wash step to zero. The elution of target proteins or complexes can be triggered under mild conditions with the biotin-containing Buffer BXT or under denaturing conditions by boiling in SDS gel loading buffer.
If you want to learn more about how to improve your yield when working with magnetic beads, please check out our guide on the optimiziation of magnetic bead-based protein purification.

Specifications

Bead Size: 30 µm (average)
Form: 5% suspension
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Specificity: Strep-tag®II and Twin-Strep-tag®
Support: 6% agarose, crosslinked, spherical magnetic beads
Twin-Strep-tag® Binding Capacity: 42.5 µg/µl

Shipping information

Storage: 2-8 °C
Stability: 12 months after shipping
Shipping: Room temperature

Accessories

Magnetic Separator
Device for separation of magnetic beads from residual solution
Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer
Buffer BXT (10x)
Strep-Tactin®XT elution buffer
Biotin
Biotin powder for preparation of Strep-Tactin®XT elution buffer

Related Products

Strep-Tactin®XT 4Flow® high capacity Spin Column Kit
For rapid purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® FPLC column
FPLC column to purify Strep-Tag®II or Twin-Strep-tag® fusion proteins