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Strep-Tactin®XT 4Flow® high capacity is perfectly suited for purifying Strep-tag®II or Twin-Strep-tag® fusion proteins. This resin offers excellent pressure stability, pH compatibility, and target purity (> 95%). Additionally, this resin is compatible with a broad range of reagents.
Its high binding affinity makes it ideal for low abundant, high-expressing and challenging proteins. A low concentration of agarose also facilitates the purification of large proteins (>90 kDa).  When working with, for example, multimeric proteins, choosing a resin with the Strep-Tactin® ligand is recommended.
Strep-Tactin®XT 4Flow® high capacity resin has a higher density of immobilized Strep-Tactin®XT than the classic Strep-Tactin®XT 4Flow®and thus can bind more protein.
As the resin is optimized for column affinity chromatography, rather than batch purification, the suspension can be used to pack gravity flow columns.

If you want more information, check out our resin specification overview.

Specifications

Bead Size: 50 - 150 µm spherical
Form: 50% suspension
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Protein Binding Capacity: 31 mg/ml resin
Specificity: Twin-Strep-tag® and Strep-tag®II
Support: 4% agarose, crosslinked

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer
Biotin
Biotin powder for preparation of Strep-Tactin®XT elution buffer
Buffer BXT (10x)
Strep-Tactin®XT elution buffer
Buffer XT-R
Strep-Tactin®XT regeneration buffer
Strep-Tactin®XT Buffer Set
Strep-Tactin®XT purification buffer set

Related Products

Strep-Tactin®XT 4Flow® high capacity Spin Column Kit
For rapid purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Empty Spin Columns
For rapid protein purification with Strep-Tactin®XT 4Flow® high capacity
MagStrep® Strep-Tactin®XT beads
Magnetic beads (5% suspension) to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity FPLC column
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® FPLC column
FPLC column to purify Strep-Tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins