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Strep-Tactin®XT 4Flow® high capacity FPLC columns contain 4% agarose resin, coupled to Strep‑Tactin®XT. The columns can be used for fully automated purification of Strep-tag®II or Twin-Strep-tag® fusion proteins with HPLC/FPLC devices, like Äkta®. The resin is suitable for all protein classes, especially low abundant or challenging proteins. The low concentration of agarose is suitable for the purification of targets with high molecular weights. In comparison to Strep-Tactin® 4Flow® resin, this high capacity variant has higher density of Strep-Tactin®XT, resulting in twice as much purified protein. The resin has broad pH compatibility, excellent pressure stability, strong binding affinity and delivers high target purity (> 95 %).

For the purification of multimeric proteins, we recommend using Strep-Tactin® 4Flow® high capacity FPLC columns instead, since the increased avidity might influence elution efficiency. If you want more information, check out our resin specification overview.

Specifications

Bead Size: 50 - 150 µm spherical
Form: Pre-packed column
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Protein Binding Capacity: 31 mg/ml resin
Specificity: Twin-Strep-tag® and Strep-tag®II
Support: 4% agarose, crosslinked

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer
Buffer BXT (10x)
Strep-Tactin®XT elution buffer
Biotin
Biotin powder for preparation of Strep-Tactin®XT elution buffer
Buffer XT-R
Strep-Tactin®XT regeneration buffer
Strep-Tactin®XT Buffer Set
Strep-Tactin®XT purification buffer set

Related Products

Strep-Tactin®XT 4Flow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity Spin Column Kit
For rapid purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® FPLC column
FPLC column to purify Strep-Tag®II or Twin-Strep-tag® fusion proteins
MagStrep® Strep-Tactin®XT beads
Magnetic beads (5% suspension) to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Empty Spin Columns
For rapid protein purification with Strep-Tactin®XT 4Flow® high capacity