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StarGate Acceptor Vector designed for high-level stable transient expression in most mammalian hosts containing the following elements:
- Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
- Episomal replication through Epstein Barr Virus replication origin (oriP) requires chromosomally expressed nuclear antigen encoded by EBNA-1
- Ampicillin resistance and ColE1 replication origin (pUC) for propagation in E. coli.
- Optimized for MEXi (Mammalian expression system IBA)
The expressed recombinant protein will be secreted in the cell culture medium (BM40 signal sequence).
This vector contains a N-terminal Twin-Strep-tag.
- Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
- Episomal replication through Epstein Barr Virus replication origin (oriP) requires chromosomally expressed nuclear antigen encoded by EBNA-1
- Ampicillin resistance and ColE1 replication origin (pUC) for propagation in E. coli.
- Optimized for MEXi (Mammalian expression system IBA)
The expressed recombinant protein will be secreted in the cell culture medium (BM40 signal sequence).
This vector contains a N-terminal Twin-Strep-tag.
| Affinity Tag N'-terminal: | Twin-Strep-tag® |
|---|---|
| Cloning Method: | Direct cloning using restriction enzyme Esp3I |
| Concentration: | 250 ng/µl |
| Expression Host: | Mammalian cells, MEXi-293E cells |
| Form: | Suspension in TE buffer |
| Possible Application: | Vector for recombinant expression in mammalian cells |
| Promoter: | CMV Promoter |
| Resistance: | Ampicillin |
| Sequence: | See Documents |
| Size: | 5874 bp |
| Storage: | -20 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
Accessories
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