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The pCSG-IBA104 vector allows high-level expression of Twin-Strep-tag®-fusion-proteins in mammalian hosts under G418 (Geneticin) selection. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins in a wide range of mammalian cells and the BM40 signal for secretion of the recombinant protein and the Twin-Strep-tag® for N-terminal fusion to the recombinant protein. The episomal replication is mediated through the Epstein Barr Virus replication origin (oriP) and nuclear antigen encoded by EBNA-1 in human, primate and canine cells and through SV40 replication origin in cells latently infected with SV40 or that express the SV40 large T antigen (e.g. COS-1, COS-7). For selection and propagation in [i]E.coli[/i] the vector carries an Ampicillin resistance and ColE1 origin of replication (pUC). The expressed recombinant protein will be secreted into the medium.
| Affinity Tag N'-terminal: | Twin-Strep-tag® |
|---|---|
| Cloning Method: | Direct cloning using restriction enzyme Esp3I |
| Concentration: | 250 ng/µl |
| Expression Host: | Mammalian cells |
| Form: | Suspension in TE buffer |
| Possible Application: | Vector for recombinant expression in mammalian cells |
| Promoter: | CMV Promoter |
| Resistance: | Ampicillin, Neomycin |
| Sequence: | See Documents |
| Size: | 11022 bp |
| Storage: | -20 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
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