2-1252-001
Strep-Tactin® 4Flow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
- New product! Receive 10% introductory discount* until 31.12.24.
Strep-Tactin® 4Flow® high capacity columns are designed for purifying Strep-tag®II and Twin-Strep-tag® proteins via affinity chromatography in gravity flow. This product features a 4% cross-linked agarose beads coupled with Strep-Tactin®, a variant of streptavidin. Strep-Tactin® selectively binds to proteins fused with a Strep-tag®II or Twin-Strep-tag® and enables targeted elution via desthiobiotin. It can be regenerated with 100 mM NaOH for multiple uses, offering a cost-effective solution. This product serves as an alternative to our Strep-Tactin®XT 4Flow® resins for proteins that are challenging to elute.
Please note that biotinylated proteins also bind to Strep-Tactin® 4Flow® high capacity. For samples containing biotinylated proteins, consider using BioLock.
For comprehensive details on our available resins, including recommendations, refer to our resin specifications.
*To redeem the discount just add the products in your shopping cart, the discount will be applied automatically. Distributors and IBA Staff excluded. Cannot be combined with other discount codes and promotions, or exchanged for monetary value. No minimum or maximum discount value. Shipping costs are excluded from discount.
Bead Size: | 50 - 150 µm spherical |
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Form: | Pre-packed gravity flow column |
Possible Application: | Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins |
Protein Binding Capacity: | 20 mg/ml resin |
Specificity: | Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin |
Support: | 4% agarose, crosslinked |
Storage: | 2-8 °C |
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Stability: | 6 months after shipping |
Shipping: | Room temperature |