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Strep-Tactin® 4Flow® high capacity columns are designed for purifying Strep-tag®II and Twin-Strep-tag® proteins via affinity chromatography in gravity flow. This product features a 4% cross-linked agarose beads coupled with Strep-Tactin®, a variant of streptavidin. Strep-Tactin® selectively binds to proteins fused with a Strep-tag®II or Twin-Strep-tag® and enables targeted elution via desthiobiotin. It can be regenerated with 100 mM NaOH for multiple uses, offering a cost-effective solution. This product serves as an alternative to our Strep-Tactin®XT 4Flow® resins for proteins that are challenging to elute. Please note that biotinylated proteins also bind to Strep-Tactin® 4Flow® high capacity. For samples containing biotinylated proteins, consider using BioLock. For comprehensive details on our available resins, including recommendations, refer to our resin specifications.

*To redeem the discount just add the products in your shopping cart, the discount will be applied automatically. Distributors and IBA Staff excluded. Cannot be combined with other discount codes and promotions, or exchanged for monetary value. No minimum or maximum discount value. Shipping costs are excluded from discount. 

Specifications

Bead Size: 50 - 150 µm spherical
Form: Pre-packed gravity flow column
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Protein Binding Capacity: 20 mg/ml resin
Specificity: Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin
Support: 4% agarose, crosslinked

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

WET FRED Set
Application aid for large volumes of lysates or culture supernatants
Buffer E (10x)
Strep-Tactin® elution buffer with desthiobiotin
Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer
Desthiobiotin
Desthiobiotin powder for preparation of Strep-Tactin® elution buffer

Related Products

MagStrep® Strep-Tactin® beads
Magnetic beads (5% suspension) to purify biotinylated proteins
Strep-Tactin® 4Flow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins - New product! Receive 10% introductory discount* until 31.12.24.
Strep-Tactin® 4Flow® high capacity FPLC column
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins - New product! Receive 10% introductory discount* until 31.12.24.