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MEXi-293E cells
HEK293 cell line for transient expression of recombinant proteins
MEXi-293E cells are human embryonic kidney (HEK) cells derived from the 293 cell line and used for propagation of plasmids and expression of recombinant proteins in mammalian cells. The cell line expresses the EBNA-1 protein for episomal replication of oriP-harboring plasmids (pDSG-IBA vectors), is adapted for growth in suspension, and for use with the MEXi cell culture medium. The cells are part of the MEXi mammalian expression system.
The MEXi system was developed to offer an affordable mammalian protein expression system coming along with all essential components:
• MEXi-293E cell line
• A variety of pDSG expression vectors
• MEXi-CM culture medium
• MEXi-TM transfection medium
• MEXi-293E cell line
• A variety of pDSG expression vectors
• MEXi-CM culture medium
• MEXi-TM transfection medium
Further advantages:
• Transient expression for time- and cost-efficient protein production
• Multiple expression vectors with different features
• Optimized for Twin-Strep-tag®:Strep-Tactin®XT system for highly pure proteins
• IP-free system
• Transient expression for time- and cost-efficient protein production
• Multiple expression vectors with different features
• Optimized for Twin-Strep-tag®:Strep-Tactin®XT system for highly pure proteins
• IP-free system
| Biosafety Level: | S1 (Germany), BSL-2 (USA) |
|---|---|
| Form: | Cryopreserved |
| Growth Properties: | Suspension culture |
| Possible Application: | Expression host for recombinat Strep-tag®II and Twin-Strep-tag® proteins |
| Storage: | -140 to -196 °C |
|---|---|
| Stability: | 6 months after shipping |
| Shipping: | Dry ice |
Accessories
pDSG-IBAwt2 vector
Mammalian expression vector for secreted proteins without affinity tag
pDSG-IBA43 vector
Mammalian expression vector encoding an N-terminal His-tag and C-terminal Strep-tag®II
pDSG-IBA45 vector
Mammalian expression vector encoding an N-terminal Strep-tag®II and C-terminal His-tag
pDSG-IBA102 vector
Mammalian expression vector for secretion of C-terminal Twin-Strep-tag® fusion proteins
pDSG-IBA103 vector
Mammalian expression vector encoding a C-terminal Twin-Strep-tag®
pDSG-IBA104 vector
Mammalian expression vector for secretion of N-terminal Twin-Strep-tag® fusion proteins
pDSG-IBA105 vector
Mammalian expression vector encoding an N-terminal Twin-Strep-tag®
Related Products
MagStrep® Strep-Tactin® beads
Magnetic beads (5% suspension) to purify biotinylated proteins
Strep-Tactin®XT 4Flow® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
