Skip to main content
Abonnieren Sie hier unseren Newsletter und erhalten Sie 10%* Rabatt auf Ihren nächsten Einkauf! (Bedingungen und Konditionen*)
Strep-Tactin® 4Flow® high capacity is a resin for purification of strep-tagged proteins via affinity chromatography. The product is optimized for column-based use and suitable for HPLC/FPLC. It consists out of cross-linked agarose beads that are coupled to Strep-Tactin®, which is a streptavidin variant. Strep-Tactin® binds to proteins that were fused to a Strep-tag®II or a Twin-Strep-tag®, enabling a straightforward purification of tagged proteins from mixed samples, reaching a purity of >95%. Due to the targeted elution, tedious titration steps are not necessary. In addition, regeneration allows for multiple use of the resin. This resin is an alternative for proteins that are difficult to elute from our Strep-Tactin®XT 4Flow® resins. Please note that biotinylated proteins also bind to Strep-Tactin® 4Flow® high capacity. If biotinylated proteins are expected, use BioLock. Furthermore, this resin is a 50% suspension, so for packing e.g. a 1 ml gravity column, 2 ml of resin is required. For further information about our different resins including recommendations, check our resin specifications.

*To redeem the discount just add the products in your shopping cart, the discount will be applied automatically. Distributors and IBA Staff excluded. Cannot be combined with other discount codes and promotions, or exchanged for monetary value. No minimum or maximum discount value. Shipping costs are excluded from discount. 

Specifications

Bead Size: 50 - 150 µm spherical
Form: 50% suspension
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Protein Binding Capacity: 20 mg/ml resin
Specificity: Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin
Support: 4% agarose, crosslinked

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer
Desthiobiotin
Desthiobiotin powder for preparation of Strep-Tactin® elution buffer
Buffer E (10x)
Strep-Tactin® elution buffer with desthiobiotin

Related Products

Strep-Tactin® 4Flow® high capacity FPLC column
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins - New product! Receive 10% introductory discount* until 31.12.24.
Strep-Tactin® 4Flow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins - New product! Receive 10% introductory discount* until 31.12.24.
Empty Spin Columns
For rapid protein purification with Strep-Tactin®XT 4Flow® high capacity