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The pASK-IBA6C vector allows the expression of [i]Strep[/i]-tag®-fusion-proteins in [i]E.coli[/i].
The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins, the ompA signal for periplasmic secretion of the recombinant protein, the [i]Strep[/i]-tag® for N-terminal fusion to the recombinant protein and the Chloramphenicol Resistance cassette. It can be used with any [i]E. coli[/i] strain because the [i]tet[/i]-promoter works independently from the genetic background of [i]E.coli[/i]. The additional factor Xa cleavage site allows the removal of the [i]Strep[/i]-tag®.
- [b]promoter[/b] from bp 37 to 72
- [b]forward primer binding site[/b] from bp 57 to 76
- [b]OmpA signal sequence[/b] from bp 139 to 201
- [b][i]Strep[/i]-tag®[/b] from bp 202 to 231
- [b]factor Xa cleavage site[/b] from bp 232 to 243
- [b]multiple cloning site[/b] from bp 244 to 320
- [b]reverse primer binding site[/b] from bp 388 to 404
- [b]f1 origin[/b] from bp 417 to 855
- [b]CamR resistance gene[/b] from bp 977 to 1636
- [b][i]tet[/i]-repressor[/b] from bp 1649 to 2272
The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins, the ompA signal for periplasmic secretion of the recombinant protein, the [i]Strep[/i]-tag® for N-terminal fusion to the recombinant protein and the Chloramphenicol Resistance cassette. It can be used with any [i]E. coli[/i] strain because the [i]tet[/i]-promoter works independently from the genetic background of [i]E.coli[/i]. The additional factor Xa cleavage site allows the removal of the [i]Strep[/i]-tag®.
- [b]promoter[/b] from bp 37 to 72
- [b]forward primer binding site[/b] from bp 57 to 76
- [b]OmpA signal sequence[/b] from bp 139 to 201
- [b][i]Strep[/i]-tag®[/b] from bp 202 to 231
- [b]factor Xa cleavage site[/b] from bp 232 to 243
- [b]multiple cloning site[/b] from bp 244 to 320
- [b]reverse primer binding site[/b] from bp 388 to 404
- [b]f1 origin[/b] from bp 417 to 855
- [b]CamR resistance gene[/b] from bp 977 to 1636
- [b][i]tet[/i]-repressor[/b] from bp 1649 to 2272
| Affinity Tag N'-terminal: | Strep-tag®II |
|---|---|
| Cloning Method: | Direct cloning using e.g. restriction enzyme BsaI in MCS |
| Concentration: | 250 ng/µl |
| Expression Host: | E. coli |
| Form: | Suspension in TE buffer |
| Possible Application: | Expression plasmid for Strep-tag®II fusion proteins |
| Promoter: | tet Promoter |
| Resistance: | Chloramphenicol |
| Sequence: | See Documents |
| Size: | 3081 bp |
| Specificity: | Factor Xa cleavage site |
| Storage: | -20 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
Accessories
Anhydrotetracycline
Tetracyclin-Derivat zur Induktion von Tetracyclin-gesteuerten Promotoren
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Periplasmic E. coli expression vector encoding a C-terminal Strep-tag®II
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