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The pASK-IBA4C vector allows the expression of [i]Strep[/i]-tag®-fusion-proteins in [i]E.coli[/i].
The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins and the ompA signal for periplasmic secretion of the recombinant protein, the [i]Strep[/i]-tag® for N-terminal fusion to the recombinant protein and the Chloramphenicol Resistance cassette. It can be used with any [i]E. coli[/i] strain because the [i]tet[/i]-promoter works independently from the genetic background of [i]E.coli[/i].
- [b]promoter[/b] from bp 37 to 72
- [b]forward primer binding site[/b] from bp 57 to 76
- [b]OmpA signal sequence[/b] from bp 139 to 201
- [b][i]Strep[/i]-tag®[/b] from bp 202 to 231
- [b]multiple cloning site[/b] from bp 232 to 313
- [b]reverse primer binding site[/b] from bp 381 to 397
- [b]f1 origin[/b] from bp 410 to 848
- [b]CamR resistance gene[/b] from bp 970 to 1629
- [b][i]tet[/i]-repressor[/b] from bp 1642 to 2265
The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins and the ompA signal for periplasmic secretion of the recombinant protein, the [i]Strep[/i]-tag® for N-terminal fusion to the recombinant protein and the Chloramphenicol Resistance cassette. It can be used with any [i]E. coli[/i] strain because the [i]tet[/i]-promoter works independently from the genetic background of [i]E.coli[/i].
- [b]promoter[/b] from bp 37 to 72
- [b]forward primer binding site[/b] from bp 57 to 76
- [b]OmpA signal sequence[/b] from bp 139 to 201
- [b][i]Strep[/i]-tag®[/b] from bp 202 to 231
- [b]multiple cloning site[/b] from bp 232 to 313
- [b]reverse primer binding site[/b] from bp 381 to 397
- [b]f1 origin[/b] from bp 410 to 848
- [b]CamR resistance gene[/b] from bp 970 to 1629
- [b][i]tet[/i]-repressor[/b] from bp 1642 to 2265
| Affinity Tag N'-terminal: | Strep-tag®II |
|---|---|
| Cloning Method: | Direct cloning using e.g. restriction enzyme BsaI in MCS |
| Concentration: | 250 ng/µl |
| Expression Host: | E. coli |
| Form: | Suspension in TE buffer |
| Possible Application: | Expression plasmid for Strep-tag®II fusion proteins |
| Promoter: | tet Promoter |
| Resistance: | Chloramphenicol |
| Sequence: | See Documents |
| Size: | 3074 bp |
| Storage: | -20 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
Accessories
Anhydrotetracycline
Tetracyclin-Derivat zur Induktion von Tetracyclin-gesteuerten Promotoren
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Periplasmic E. coli expression vector encoding a C-terminal Strep-tag®II
pASG-IBA4 vector
Periplasmic E. coli expression vector encoding an N-terminal Strep-tag®II
