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StrepMAB-Immo is a murine monoclonal antibody with a high affinity to Strep-tag® and Twin-Strep-tag® fusion proteins. It is suited for the immobilization of tagged proteins on solid phases (e.g. SPR sensor chips), as well as the detection in applications like ELISAFACSimmunohistochemistry or immunocytochemistry.
Strep-tag® proteins with an N-terminal Serine-Alanine linker (protein-SA-WSHPQFEK or SA-WSHPQFEK-protein) are validated for nearly irreversible binding. Proteins with other N-terminal sequences bind with a reduced affinity. The Twin-Strep-tag® always carries the N-terminal SA linker (WSHPQFEK-GGGS-GGGS-GGSA-WSHPQFEK).
For detection of Strep-tag®II or Twin-Strep-tag® fusion proteins in Western Blots, we recommend using StrepMAB-Classic or Strep-Tactin® conjugates.

Specifications

Clonality: Monoclonal
Form: Lyophilized powder
Possible Application: ELISA, FACS, Immunofluorescence, SPR
Reconstitution: PBS
Specificity: Strep-tag®II and Twin-Strep-tag®
Subclass: IgG1
Species: Mouse

Shipping information

Storage: -20 °C
Stability: 24 months after shipping
Shipping: Room temperature

Accessories

GFP-Strep-tag®II control protein
Control protein for Strep-Tactin® and Strep-Tactin®XT applications
GFP-Twin-Strep-tag® control protein
Control protein for Strep-Tactin® and Strep-Tactin®XT applications

Related Products

StrepMAB-Classic
Monoclonal antibody to detect Strep-tag®II and Twin-Strep-tag® fusion proteins
Strep-Tactin®
Reversible immobilization of biotinylated analytes or Strep-tag®II and Twin-Strep-tag® fusion proteins
Twin-Strep-tag® Capture Kit
Reversible capture of Twin-Strep-tag® fusion proteins for SPR analysis
StrepMAB-Immo DY-488
Conjugated monoclonal antibody to detect Strep-tag®II and Twin-Strep-tag® fusion proteins
StrepMAB-Immo DY-549
Conjugated monoclonal antibody to detect Strep-tag®II and Twin-Strep-tag® fusion proteins
StrepMAB-Immo DY-649
Monoclonal antibody conjugated to DY-649 to detect Strep-tag®II and Twin-Strep-tag® fusion proteins