2-4150-025

Strep-Tactin®XT Spin Columns

For rapid purification of Twin-Strep-tag and Strep-tag®II fusion proteins from small samples

€133.00*

Content
Strep-Tactin®XT Spin Columns contain dried polymethacrylate, a synthetic material coupled with the streptavidin variant Strep-Tactin®XT and are suitable for purification of Strep-tag®II or Twin-Strep-tag® fusion proteins. The pre-packed spin columns are provided with receiver tubes and compatible with conventional microcentrifuges, allowing a rapid, parallel processing of multiple samples.
Up to 400 µg of mCherry-Strep-tag®II (28 kDa) can be purified under physiological conditions in less than 10 minutes. Prior to application, the spin columns need to be re-hydrated and activated with Buffer W. In contrast to agarose resins, plant cell components such as chlorophyll are not unspecifically bound by the resin, making Strep-Tactin®XT Spin Columns a favorable alternative.
Please note that the spin columns are not reusable.

Specifications

Form: 25 pre-packed spin columns with receiver tubes
Kit Content: 25 spin columns and receiver tubes
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Specificity: Twin-Strep-Tag® and Strep-tag®II
Support: Polymethacrylate
Twin-Strep-tag® Binding Capacity: 400 µg/spin column

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

10x Buffer W
Strep-Tactin® and Strep-Tactin®XT wash buffer
10x Buffer BXT
Strep-Tactin®XT elution buffer
Biotin
Biotin powder for preparation of Strep-Tactin®XT elution buffer

Related Products

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Strep-Tactin®XT 4Flow® column
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Strep-Tactin®XT 4Flow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT Spin Column Kit
All reagents for rapid purification of Twin-Strep-tag® and Strep-tag®II fusion proteins