2-5027-001

Strep-Tactin®XT 4Flow® high capacity cartridge

HPLC/FPLC cartridge to purify Strep-tag®II or Twin-Strep-tag® fusion proteins

€105.00*

Content

Please be informed that we have changed Cat. No. 2-5025-001 and 2-5026-001 to 2-5027-001 and 2-5028-001 due to a change in the cartridge body. There are no differences in the specifications or performance of these new cartridges so that they can be used as before.

Strep-Tactin®XT 4Flow® high capacity cartridges contain a 4% agarose coupled with the streptavidin variant Strep-Tactin®XT and are applicable for purification of Strep-tag®II or Twin-Strep-tag® fusion proteins with HPLC/FPLC devices like Äkta® systems (Cytiva). Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (> 95%), and highest binding affinity, the resin is recommended for all protein classes, especially for low abundant or challenging proteins. The low concentrated agarose enables the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets. In addition, Strep-Tactin®XT 4Flow® allows the application of denaturing agents such as urea. In comparison to Strep-Tactin®XT 4Flow®, Strep-Tactin®XT 4Flow® high capacity has a higher density of immobilized Strep-Tactin®XT, which leads to a 2-fold higher yield of purified protein.

Specifications

Bead Size: 50 - 150 µm spherical
Dynamic Protein Binding Capacity: 14 mg/ml resin
Form: Pre-packed cartridge
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Specificity: Twin-Strep-Tag® and Strep-tag®II
Support: 4% agarose, crosslinked

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

10x Buffer W
Strep-Tactin® and Strep-Tactin®XT wash buffer
10x Buffer BXT
Strep-Tactin®XT elution buffer
Biotin
Biotin powder for preparation of Strep-Tactin®XT elution buffer
Buffer XT-R
Strep-Tactin®XT regeneration buffer
Strep-Tactin®XT Buffer Set
Strep-Tactin®XT purification buffer set

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Strep-Tactin®XT 4Flow® high capacity resin
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Strep-Tactin®XT 4Flow® cartridge
HPLC/FPLC cartridge to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins