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Strep-Tactin® Magnetic Microbeads
Strep-Tactin® multimers conjugated with magnetic microbeads
Please note: Strep-Tactin® Magnetic Microbeads were specifically developed for cell isolation. For protein purification, we offer MagStrep® Strep-Tactin®XT beads , which have an agarose coating that guarantees low binding of unspecific proteins and ensures a high protein binding capacity.
Strep-Tactin® Magnetic Microbeads are microspheres covalently linked to Strep-Tactin®. They are suitable for selecting cells that express a Strep-tag®II or Twin-Strep-tag® - fusion protein on the surface. Another approach is the isolation of cells via a strep-tagged protein that specifically binds to a target cell of interest. This can be e.g. an antigen for B cell selection, an MHC class I molecule for T cell selection or any type of antibody for a cell of choice. For this approach, Strep-Tactin® Magnetic Microbeads have to be complexed with a strep-tagged protein to generate a selection reagent. After the cells are isolated, biotin addition will cause the release of Strep-Tactin® Magnetic Microbeads from the cell surface.
| Bead Size: | 1 - 3 µm |
|---|---|
| Detector/Ligand: | Strep-Tactin® |
| Form: | Suspension in DPBS |
| Format: | Magnetic beads |
| Possible Application: | Cell isolation (magnetic) |
| Recommended Usage: | 15 µl per 1 x 10^7 total cells |
| Storage: | 2-8 °C; Do not freeze! |
|---|---|
| Stability: | 6 months after shipping |
| Shipping: | Room temperature |
Accessories
Biotin stock solution
100 mM concentrated biotin for dissociation of Twin-Strep-tag® from Strep-Tactin® suitable for cells
StrepMan Magnet
Permanent magnet for cell isolations using Strep-Tactin® Magnetic Microbeads
