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Strep-Tactin® APC
Fluorophore-conjugated Strep-Tactin® for cell staining
Content
Strep-Tactin® conjugated to allophycocyanin (APC) binds to Strep-tag®II- or Twin-Strep-tag®- fusion proteins and is optimized for flow cytometry of cells. Strep-Tactin® is a streptavidin variant and therefore it is a tetrameric molecule that also has an affinity to biotin.
Cells can either be stained directly if they express a strep-tagged surface protein or indirectly via a strep-tagged protein that subsequently binds to the cells (e.g. for sorting antigen-specific B cells).
Independent from the chosen method, biotin addition will cause the release of Strep-Tactin® APC from the cell surface. This enables the use of selected cells for further downstream applications.
Cells can either be stained directly if they express a strep-tagged surface protein or indirectly via a strep-tagged protein that subsequently binds to the cells (e.g. for sorting antigen-specific B cells).
Independent from the chosen method, biotin addition will cause the release of Strep-Tactin® APC from the cell surface. This enables the use of selected cells for further downstream applications.
Specifications
| Concentration: | 75 µg/ml |
|---|---|
| Form: | Suspension in DPBS |
| Possible Application: | Fluorescent cell staining & sorting |
| Specificity: | Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin |
Shipping information
| Storage: | 2-8 °C; Do not freeze! |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Blue ice |
Accessories
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Biotin stock solution
100 mM concentrated biotin for dissociation of Twin-Strep-tag® from Strep-Tactin® suitable for cells
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Strep-Tactin® TACS Agarose Column
Gravity flow column suitable for cell and exosome isolation via affinity chromatography
Strep-Tactin® TACS Agarose
50% suspension for isolation of cells and exosomes via affinity chromatography
