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For the specific detection of Strep-tag® and Twin-Strep-tag® fusion proteins, Strep-Tactin® is available conjugated to alkaline phosphatase (AP). The proteins can be captured on a membrane or microplate. After application of Strep-Tactin® AP, they can be visualized in a chromogenic reaction.
Strep-Tactin® AP cross-reacts with biotin and biotinylated proteins (e.g. the biotin carboxyl carrier protein in E. coli). To prevent the detection of false positives, use 2.5 mg/ml avidin for biotin masking. 

Specifications

Amount: 500 µl
Form: Suspension in PBS
Possible Application: Western blot, detection in microplates
Specificity: Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin

Shipping information

Storage: 2-8 °C
Stability: 12 months after shipping
Shipping: Room temperature

Accessories

GFP-Strep-tag®II control protein
Control protein for Strep-Tactin® and Strep-Tactin®XT applications
Strep-tag®II peptide
Synthetic peptide recognized by Strep-Tactin® and Strep-Tactin®XT

Related Products

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StrepMAB-Classic DY-488
Conjugated monoclonal antibody to detect Strep-tag®II and Twin-Strep-tag® fusion proteins
StrepMAB-Classic
Monoclonal antibody to detect Strep-tag®II and Twin-Strep-tag® fusion proteins