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Strep-Tactin® Sepharose® is used preferentially for gravity flow purification of recombinant Strep-tag® proteins. Bulk resin is recommended for packing of columns since the resin is optimized for column affinity chromatography rather than for batch purification.
Binding capacity: 1 ml sedimented resin (corresponding to 2 ml of a 50% suspension) is useful for the one-step purification of 50 to 100 nmol recombinant Strep-tag® protein (up to 3 mg in case of a 30 KDa protein).
Binding capacity: 1 ml sedimented resin (corresponding to 2 ml of a 50% suspension) is useful for the one-step purification of 50 to 100 nmol recombinant Strep-tag® protein (up to 3 mg in case of a 30 KDa protein).
| Bead Size: | 45 - 165 µm |
|---|---|
| Biotin Binding Capacity: | > 300 nmol/ml resin |
| Exclusion Limit: | 3 x 10^7 |
| Form: | 50% suspension |
| Possible Application: | Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins |
| Specificity: | Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin |
| Strep-tag®II Binding Capacity: | 3 mg/ml |
| Support: | 4% agarose |
| Storage: | 2-8 °C |
|---|---|
| Stability: | 6 months after shipping |
| Shipping: | Room temperature |
Accessories
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Strep-Tactin® Sepharose® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity cartridge
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® MacroPrep® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
