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The pYSG-IBA5 vector allows the high-level expression of [i]Strep[/i]-tag®-fusion-proteins in yeast. The vector carries the copper inducible promoter (CUP1) for controlled high-level expression, the [i]Strep[/i]-tag® for N-terminal fusion of the recombinant protein as well as the URA3 auxotrophy marker for selection after transformation (do not use URA3 for selection during expression), the LEU2d auxotrophy marker for selection to increase plasmid copy number for expression (do not use LEU2d for selection after transformation). The expressed recombinant protein will be localized in the cytoplasm.
| Affinity Tag N'-terminal: | Strep-tag®II |
|---|---|
| Cloning Method: | Direct cloning using restriction enzyme Esp3I |
| Concentration: | 250 ng/µl |
| Expression Host: | Yeast |
| Form: | Suspension in TE buffer |
| Possible Application: | Vector for recombinant expression in yeast |
| Promoter: | CUP1 Promoter |
| Resistance: | Ampicillin |
| Sequence: | See Documents |
| Size: | 7853 bp |
| Storage: | -20 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
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