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The pLSG-IBA3 vector allows the high-level expression of [i]Strep[/i]-tag®-fusion-proteins in insect cells. The vector carries the Polyhedrin promoter for high-level expression in insect cells and the [i]Strep[/i]-tag® for C-terminal fusion to the recombinant protein. The Co-transfection with BacPAK6 linearized AcMNPV DNA (Clontech) or with circular flashBAC modified AcMNPV DNA (Oxford Expression Technologies) allows the generation of recombinant baculovirus at very high efficiency through reconstitution of an essential gene (ORF 1629) and elimination of wild-type virus to great extent. For selection and propagation in [i]E.coli[/i] the vector carries an Ampicillin resistance and ColE1 origin of replication (pUC). The expressed recombinant protein will be localized in the cytoplasm.
| Affinity Tag C'-terminal: | Strep-tag®II |
|---|---|
| Cloning Method: | Direct cloning using restriction enzyme Esp3I |
| Concentration: | 250 ng/µl |
| Expression Host: | Insect cells |
| Form: | Suspension in TE buffer |
| Possible Application: | Vector for recombinant expression in insect cells |
| Promoter: | Polyhedrin Promoter |
| Resistance: | Ampicillin |
| Sequence: | See Documents |
| Size: | 6179 bp |
| Storage: | -20 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
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