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The pESG-IBA45 vector allows the expression of [i]Strep[/i]-/6x[i]Histidine[/i]-tag-fusion-proteins in Mammalia. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins, the [i]Strep[/i]-tag® for N-terminal and the 6xHistidine-tag for C-terminal fusion to the recombinant protein as well as the Neomycin resistance gene for selection of stable cell lines. The expressed recombinant protein will be localized in the cytoplasm.
| Affinity Tag C'-terminal: | 6xHis |
|---|---|
| Affinity Tag N'-terminal: | Strep-tag®II |
| Cloning Method: | Direct cloning using restriction enzyme Esp3I |
| Concentration: | 250 ng/µl |
| Expression Host: | Mammalian cells |
| Form: | Suspension in TE buffer |
| Possible Application: | Vector for recombinant expression in mammalian cells |
| Promoter: | CMV Promoter |
| Resistance: | Ampicillin, Neomycin |
| Sequence: | See Documents |
| Size: | 6124 bp |
| Storage: | -20 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
Accessories
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