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The pASG-IBA145 vector allows the expression of Twin-Strep®-/6x[i]Histidine[/i]-tag-fusion-proteins in [i]E.coli[/i]. The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins, the Twin-Strep-tag® for N-terminal and the 6x[i]Histidine[/i]-tag for C-terminal fusion to the recombinant protein as well as the Ampicillin Resistance cassette. It can be used with any [i]E. coli[/i] strain because the tet-promoter works independently from the genetic background of [i]E.coli[/i].
The expressed recombinant protein will be localized in the cytoplasm.
The expressed recombinant protein will be localized in the cytoplasm.
| Affinity Tag C'-terminal: | 6xHis |
|---|---|
| Affinity Tag N'-terminal: | Twin-Strep-tag® |
| Cloning Method: | Direct cloning using restriction enzyme Esp3I |
| Concentration: | 250 ng/µl |
| Expression Host: | E. coli |
| Form: | Suspension in TE buffer |
| Possible Application: | Vector for recombinant expression in E. coli |
| Promoter: | tet Promoter |
| Resistance: | Ampicillin |
| Sequence: | See Documents |
| Size: | 3973 bp |
| Storage: | -20 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
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